@article{Murakawa_Hinz_Mothes-post_regul_mRNA-2015,
author = {Murakawa, Yasuhiro and Hinz, Michael and Mothes, Janina and 
          Schuetz, Anja and Uhl, Michael and Wyler, Emanuel and 
          Yasuda, Tomoharu and Mastrobuoni, Guido and Friedel, 
          Caroline C. and Dolken, Lars and Kempa, Stefan and 
          Schmidt-Supprian, Marc and Bluthgen, Nils and Backofen, Rolf 
          and Heinemann, Udo and Wolf, Jana and Scheidereit, Claus and 
          Landthaler, Markus},
title = {{RC3H1} post-transcriptionally regulates {A20} {mRNA} and 
         modulates the activity of the {IKK}/{NF}-{kappaB} pathway},
journal = {Nat Commun},
year = {2015},
doi = {10.1038/ncomms8367},
volume = {6},
user = {backofen},
pmid = {26170170},
pages = {7367},
number = {},
issn = {2041-1723},
abstract = {The RNA-binding protein RC3H1 (also known as ROQUIN) 
            promotes TNFalpha mRNA decay via a 3'UTR constitutive decay 
            element (CDE). Here we applied PAR-CLIP to human RC3H1 to 
            identify approximately 3,800 mRNA targets with >16,000 
            binding sites. A large number of sites are distinct from the 
            consensus CDE and revealed a structure-sequence motif with 
            U-rich sequences embedded in hairpins. RC3H1 binds 
            preferentially short-lived and DNA damage-induced mRNAs, 
            indicating a role of this RNA-binding protein in the 
            post-transcriptional regulation of the DNA damage response. 
            Intriguingly, RC3H1 affects expression of the NF-kappaB 
            pathway regulators such as IkappaBalpha and A20. RC3H1 uses 
            ROQ and Zn-finger domains to contact a binding site in the 
            A20 3'UTR, demonstrating a not yet recognized mode of RC3H1 
            binding. Knockdown of RC3H1 resulted in increased A20 
            protein expression, thereby interfering with IkappaB kinase 
            and NF-kappaB activities, demonstrating that RC3H1 can 
            modulate the activity of the IKK/NF-kappaB pathway.}
}