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antaRNA sequence constraint can be specified by all, specific and ambigeous IUPAC defined nucleotide constraints:
| A | Adenine |
| C | Cytosine |
| G | Guanine |
| T/U | Thymine/Uracil |
| R | A or G |
| Y | C or T/U |
| S | G or C |
| W | A or T/U |
| K | G or T/U |
| M | A or C |
| B | C or G or T/U |
| D | A or G or T/U |
| H | A or C or T/U |
| V | A or C or G |
| N | A or C or G or T/U |